Shubham Upadhayay

Research

• Senior Research Fellowship (SRF) awarded by Indian Council of Medical Research (ICMR), for the project entitled “Neuropharmacological investigation on raloxifene and fulvestrant against haloperidol-induced animal model of Tardive Dyskinesia: Possible role of GPR30 receptor”

Animal Model Validated & Developed

• Haloperidol-Induced Tardive Dyskinesia model in Adult Wistar Rats and in Zebrafish
• 3-Nitropropionic acid (3-NP) induced Huntington’s disease in adult zebrafish.
• Mercury-Induced Amyotrophic Lateral Sclerosis in Adult Wistar Rats
• Ethidium Bromide Induced Multiple Sclerosis Model in Adult Wistar Rats

Target explored in neurological disorders

GPER1/GPR30, PI3k/Akt, Nrf2/HO-1, GSK-3β, c-JNK/p38MAPK

Drug Induced Movement Disorder

• Raloxifene and Fulvestrant Exert Antioxidant, Anti-Inflammatory, and Antiapoptotic Action Against Haloperidol-Induced Tardive Dyskinesia in Rats via Activation of the GPER1/PI3k/Akt/Nrf2/HO-1 Signaling Pathways. Journal of biochemical and molecular toxicology, 39(8), e70413.

• G-Protein-Coupled Estrogen Receptor 1 (GPER1) Activation Mitigates Haloperidol-Induced Neurotoxicity in SHSY-5Y Cells and Improves Motor Functions in Adult Zebrafish. Neurochemical research, 50(2), 119.

• Glycyrrhizic Acid Mitigates Haloperidol-Induced Neurotoxicity in SHSY-5Y Cells and Rats Via Activation of PI3k/Akt/Nrf2 Pathways. Neurochemical Research, 50(1), 1-19.

• Bacillus Calmette–Guérin Vaccine Attenuates Haloperidol-Induced TD-like Behavioral and Neurochemical Alteration in Experimental Rats." Biomolecules 13, no. 11 (2023): 1667.

Huntington’s Disease and Parkinson’s Disease

• Auranofin-loaded chitosan-lipid hybrid nanoparticle protects against the in-vitro/in-vivo model of Parkinson’s disease via modulation of GSK-3β/Nrf2/HO-1 signaling. European Journal of Pharmacology, 177523.

• Elucidating the neuroprotective potential of arbutin in 3-NPA induced HD-like pathology: Insights from in silico, in vitro, and in vivo models. Behavioural Brain Research, 483, 115475.

Multiple Sclerosis

• Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis." Genes 13, no. 8 (2022): 1324

• Neuroprotection by solanesol against ethidium bromide-induced multiple sclerosis-like neurobehavioral, molecular, and neurochemical alterations in experimental rats, Phytomedicine Plus, Volume 1, Issue 4, 2021,100051

• Inhibition of c-JNK/p38MAPK signaling pathway by Apigenin prevents neurobehavioral and neurochemical defects in ethidium bromide-induced experimental model of multiple sclerosis in rats: Evidence from CSF, blood plasma and brain samples. Phytomedicine Plus 1, no. 4 (2021): 100139.

Alzheimer’s Disease

• In vitro and in vivo investigations of chromone derivatives as potential multitarget-directed ligands: Cognitive amelioration utilizing a scopolamine-induced zebrafish model. ACS Chemical Neuroscience, 15(14), 2565-2585.
• ML-based prediction to experimental validation: Development of dihydroquinazoline based multi-potent ligands as anti-Alzheimer's agents. Computers in Biology and Medicine, 196, 110762.