Personal Information

Dr. Gajendra Singh

Assistant Professor
Department of Biotechnology

M.Sc.  in Biotechnology, Dept. of Biotechnology, Rajasthan University (2011). CSIR NET JRF(2011). Ph.D. CSIR-Central Drug Research Institute, Lucknow 2017.Postdoctoral Research: New York University (2017-2018), NCBS-TIFR (2018-2023)

gajendra.singh@curaj.ac.in

  • NMR Spectroscopy,

  • Protein Biophysics,

  • Ubiquitin, Ubls, PTMs, 

  • PROTAC(Proteolysis Targeting Chimera).

2006-2009

B.Sc.  Department of sciences, M.N institute of applied sciences, Bikaner, Rajasthan, India

2009-2011

M.Sc.  in Biotechnology, Dept. of Biotechnology, Rajasthan University Dept. Jaipur, India

2012-2017

Ph.D.  Academy of Scientific and Innovative Research

(CSIR-Central Drug Research Institute, Lucknow,)

2017-2018

Postdoctoral Fellow,Dept of Chemistry, New York University, New York, USA

2018-2023

Postdoctoral Fellow, National Centre for Biological Science- Tata Institute of Fundamental Research, Bengaluru, India.

Jan-2023 Onwards

Assistant Professor, Dept. Of Biotechnology School of Life Science, Central University of Rajasthan Ajmer

My lab encompasses several areas of research including biochemistry, molecular biology and protein folding with a particular focus on the application of NMR spectroscopy, Mass Spectrometry and other biophysical techniques. One area of interest is the regulation of protein function through post-translational modifications (PTMs) such as ubiquitin, Ubls, and target protein modification. The lab aims to uncover the molecular mechanisms of PTMs and how they impact protein structure, stability, and interactions. The lab also investigates PROTAC (Proteolysis Targeting Chimera) as a potential therapeutic strategy to selectively degrade target proteins implicated in various diseases. This work involves the design, and characterization of PROTAC molecules as well as the investigation of their mechanism of action at the molecular level. Through these studies, the lab aims to gain insights into the underlying molecular mechanisms of cellular processes and identify potential targets for therapeutic intervention. 

Our lab is also  interested in exploring the conformational properties of peptides and their potential as drug targets. Peptides are versatile molecules that play essential roles in many biological processes, and their conformational flexibility allows them to interact with a wide range of targets. We use a combination of experimental and computational techniques to study the conformational properties of peptides, including NMR spectroscopy, and molecular dynamics simulations. By understanding the conformational properties of peptides, we aim to identify potential drug targets and design small molecule drugs that can modulate their activity. Ultimately, our research aims to contribute to the development of new therapeutics for a wide range of diseases.

S. No. Authors Title of Article Journal/Conference Details Journal/Conference Publication Year
1 • Gajendra Singh, U Ghosh, S Pal, RS Ampapathi, TK Chakraborty Βγ-fused turn structures in sugar amino acid (SAA) containing cyclic tetrapeptides with α3δ architecture Tetrahedron, 70, 7681-7685, 10, 2014 Journal 2014

2.    Srinivas Samala, Pragyan Pallavi, Ravi Kumar, Rajesh K Arigela, Gajendra Singh, Dr. Ravi Sankar Ampapathi, Amulya Priya, Dr. Sunando Datta, Dr. Abhijit Patra*, Dr. Bijoy Kundu* One‐Pot Synthesis of Highly Fluorescent Pyrido [1, 2‐a] indole Derivatives through C H/N  H Activation: Photophysical Investigations and Application in Cell Imaging, Chemistry–A European Journal, 20, 14344-14350, 10, 2014.

3.   S Samala, Gajendra Singh, R Kumar, RS Ampapathi, B Kundu, Metal‐Free Decarboxylative Cyclization/Ring Expansion: Construction of Five‐, Six‐, and Seven‐Membered Heterocycles from 2‐Alkynyl Benzaldehydes and Cyclic Amino Acids, Angewandte Chemie International Edition, 54, 9564-9567, 8, 2015.

4.   S Pal, Gajendra Singh, S Singh, JK Tripathi, JK Ghosh, S Sinha, RS Ampapathi, TK Chakraborty  Tetrahydrofuran amino acid-containing gramicidin S analogs with improved biological profiles, Organic & biomolecular chemistry, 13, 6789-6802, 5,  2015.

5.  Geeta Karki, Harikesh Kumar, Gajendra Singh, Ravi S Ampapathi, Pintu Kumar Mondal, Synthesis of the pentasaccharide moiety of starfish asterosaponin luidiaquinoside and its conformational analysis, RSC Adv, 6, 7736–7745, 1, 2016.

6. Rafat Ali, Gajendra Singh, Shalini Singh, Ravi Sankar Ampapathi, W Haq. Diastereoselective Synthesis of 5-Heteroaryl-Substituted Prolines Useful for Controlling Peptide-Bond Geometry, ACS Org. Lett., 18, 2848-2851, 5, 2016.

7.  S Singh, R Shrivastava, Gajendra Singh, R Ali, S Bhadhuria, RS Ampapathi, W Haq, AzaGly Appended Peptidomimetics Structurally Related to PTR6154 as Potential PKB/Akt Inhibitors, ChemBioChem, 18, 6, 1061-1065, 2017

8. Uttam Ghosh, Gajendra Singh, Sudip Pal, Faiyaz Alam, Shyam Singh, Sidharth Chopra, Sudhir Sinha, Ravi Sankar Ampapathi, and Tushar Kanti Chakraborty, Synthesis, conformational studies and biological profiles of tetrahydrofuran amino acid containing cationic antitubercular peptides, Asian Journal of Organic Chemistry, 6, 1240-1249, 9,  2017.

9.   M. Shyam Sundar, Gajendra Singh, Ravi S. Ampapathi Ashutosh, V. Bedekar, Conformational study and stereodynamics of ortho -substituted ortho -terphenyl and its derivatives, Journal of Molecular Structure, 1147, 495-501,6, 2017.

10.  Arpita Chakraborty, Soumi Biswas, Gajendra Singh, Swastik Mondal, Semanti Ghosh, Angshuman Bagchi, Ravi S. Ampapathi, and Jishu Naskar, A Terminally Capped Synthetic, Acyclic Tripeptide Forms Dimer in the Solid, Liquid and Gaseous States,Satyabrata Samui, Chemistry SELECT, 3, 2523-2527, 3, 2018.

11.  Gajendra Singh, Sarfuddin Azmi, Jimut Kanti Ghosh, Ravi Sankar Ampapathi,Sudip Pal, Synthesis and Conformational Studies of Taa-Containing o-Nitrobenzenesulfonamide- (o-Nosyl-) Protected GS Analogs to Prove the Importance of 6R Stereochemistry of Taa over 6S, Chemistry SELECT, 3, 2272-2276, 3, 2018.

12. Vinay Shankar Tiwari, Gajendra Singh, Ravi Sankar Ampapathi and Wahajul Haq, Pyrrolidine Ring Puckering and Prolyl Amide Bond Configurations of 2-methyl-allo-hydroxyproline-based dipeptides. Org. Biomol. Chem., 2019,17, 4460-4464.

13. Design, Synthesis and Conformational Studies of Cyclic Tetrapeptides having βγ Fused Turns as HDAC Inhibitors. Dr. Uttam Ghosh,Moumita Basu,Sudip Pal,Sanjeev Meena,Dipak Datta,Ravi Sankar Ampapathi,Tapas K Kundu, Gajendra Singh*,Prof. Tushar Kanti Chakraborty* Chemistry Select 6 (31), 7887-7893

14. Conformation Based In silico Studies of Cyclic tetrapeptides having βγ Fused Turns as Thrombin Inhibitors Uttam Ghoshh, [a] Vikash Kumar,Gajendra Singh,*  and Tushar Kanti Chakraborty*  2023( Chemistry Select 2023).

15. Decoding the assembly of Mixed and Branched heterotypic Ubiquitin chains. Gajendra Singh, Sanjeev Kumar and Ranabir Das1* ( Anal. Chem. 2023, 95, 26, 10061–10067)

                                                                                                   Patent

SMAC MIMETICS FOR TREATMENT OF CANCER, PROCESS FOR PREPARATION AND PHARMACEUTICAL COMPOSITION THEREOF. HAQ, WahajulALI, Rafat SINGH, Akhilesh NENGROO, Mushtaq Ahmad, KATEKAR, Roshan, SINGH Gajendra, VAISHNAV, Jayanti, AFSAR, Mohammad, SINGH, Manohar RATH, Srikanta Kumar, KOLEY, Dipankar MISHRA, Durga Prasad, RAMACHANDRAN, Ravishankar, AMPAPATHI, Ravi Sankar, GAYEN, Jiaur Rahaman, DATTA Dipak.Publication NumberWO/2022/130411.Publication Date23.06.202

1. Structural-functional analysis of ubiquitin-conjugating enzyme E2-25K -Huntington-interacting protein 2 (DST-SERB, 2019-2022 Completed)

Attended

Conferences/ Seminars

1. Attended 11th International conference BIOCON-11, on Innovation in the Latest Healthcare Issue at Biyani Institute of Science & Management Jaipur. Conducted by Department of Biotechnology Govt. of India.

2. Poster presentation in the 5th international symposium on the “Drug Development for the Orphan /Neglected Disease “at CSIR-Central Drug Research, Lucknow from 26th to 28th February 2013

3. Poster presentation in “20th National Magnetic Resonance Society Symposium cum Annual meeting –NMRS-2014” at Department of Chemical science, Tezpur University, India from 2-5 February 2014.

4. Poster presentation in “Drug Discovery in India: The Past, Present and Future “at CSIR-Central Drug Research, Lucknow 1st January 2015  

5. Poster presentation in “22nd Conference of National Magnetic Resonance Society –NMRS 2016” organized by the Department of Chemistry, Indian Institute of Technology Kharagpur, India 18th -21st February 2016

6. Participate in the 6th International Symposium on “Current Trends in Drug Discovery and Research CTDDR-2016” at Central Drug Research Institute Lucknow, 25th -28th February 2016.

7. Participated in the India International Science Festival – 2016 “Young Scientist Conclave “at CSIR-NPL Delhi, Organized by the Department of Science and Technology, Govt. of India .7th -11th December 2016.

8. Participate in the 4th International Symposium on “NMR Biology Meet” at Khajuraho, 16th -21th December 2018

 

Awards

1. Incentive awards for the publication 2016 in Angewandte Chemie-International Edition 54(33)9564-9567 by the CSIR-CDRI Lucknow India

2. Incentive awards for the publication 2017 in ACS Organic letters 18(12), 2848-2851   by the CSIR-CDRI Lucknow India

3. Lifetime Member of National Magnetic Resonance society (NMRS), India 

4. Member of New York academy of science (2018-2019)

5. Member of International Society of Magnetic Resonance (ISMAR)

6. Affiliate member of the Royal Society of Chemistry.

7. Member of " The Science Advisory Board" Arlington Texas United States.